![]() The method of claim 1, wherein the fixturing plate is placed between the skin of the subject and the outside lip of the window frame to stabilize the tissue of interest, wherein the fixturing plate has a recess for holding the window frame, and wherein the fixturing plate is secured to the microscope stage.ġ2. The method of claim 1, wherein the window frame is made of metal and/or plastic.ġ1. The method of claim 7, wherein the tissue is a mammary fat-pad, lymph node or salivary gland.ġ0. The method of claim 7, wherein the rigid backing is made of one or more of rubber, plastic and metal.ĩ. The method of claim 1, wherein additional stabilization of the tissue of interest is provided by affixing a rigid backing to a side of the tissue of interest opposite to the objective lens of the microscope.Ĩ. The method of claim 1, comprising attaching the window frame to the tissue of interest and applying saline within the window frame before the window is affixed to the window frame to cover the tissue of interest with saline.ħ. The method of claim 1, wherein the window frame is attached to the subject using adhesive.Ħ. ![]() The method of claim 1, wherein the fixturing plate is made of metal and/or plastic.ĥ. The method of claim 1, wherein the fixturing plate comprises portions defining a slotted recess that holds the window frame.Ĥ. The method of claim 1, wherein the fixturing plate comprises portions defining a circular hole for the window and a circular recess that holds the window frame.ģ. A method of high-resolution intravital imaging of a tissue of interest in a subject using a microscope comprising: a) exposing the tissue of interest in the subject, b) attaching a window frame holding a transparent window to the subject so that the tissue of interest is viewable through the window, c) attaching the window frame to a microscope stage with a window fixturing plate that is movable relative to the optical axis of the microscope in at least x- and y-directions, and d) acquiring through the window high-magnification, high-resolution overlapping images of the tissue of interest in a mosaic pattern and stitching the images together to produce a low-magnification, high-resolution overview image of the tissue of interest that preserves subcellular resolution, thereby obtaining high-resolution intravital images of a tissue of interest in a subject.Ģ. ![]() It is nice to not have to nuke and then cool down overly heated wax-like heating pads or fold a normal heating pad into a ridiculously small space.1. I don't use it as much as I maybe should, but we do use it as a heating pad during surgery (calibrating the temp of the pad, not by the rectal probe). Very prompt in attention and getting it fixed and returned.Ĥ. When we have had problems (and we have had problems, and my husband lab had a problem), you guys are on it. My university leaves me alone about my unit because it isn't a traditional unit and doesn't require service/calibration unless we've noticed a problem.ģ. It is a pain to send off these units which are essential to lab progress once a year to get them serviced and calibrated. Also, now not a problem with our annual safety evaluation since we don't have compressed cylinders.Ģ. This is nice as inevitably someone in lab fails to let others know that we're almost out. Sharpe, PhD - University of Oklahoma Health Sciences Center - College of Pharmacy Oklahoma, OKġ. No hot spots like you see with traditional warming pads.įIR warming increases body metabolism, thereby discharging anesthetics and toxins, which aid in faster recovery from surgery.Īmanda L. Your animal can now be warmed over an extended period of time without having to constantly increase the temperature. FIR allows researchers to safely warm animals for a longer period of time without unknowingly overheating the animal. Less mouse warming is required with FIR than with conventional techniques. Studies have shown that FIR can maintain therapeutic temperatures longer when compared with conventional heating methods. The body can absorb as much as 90% of FIR heat compared to only 20% with conventional warming pads. The energy level of water in the body's cells is gently increased through “resonant absorption” warming the inside of the body. FIR waves resonate with organic matter and penetrate deep into the body. FIR is totally safe in fact, 80% of the sun’s rays are in the low energy, far infrared range. FIR light warms the body without warming the air between the FIR source and the body. Far infrared light is absorbed by cells deep within the body of the animal which safely raise the core body temperature. The pads warm the animal through far infrared (FIR) warming.
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